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inverted phase contrast microscope  (Nikon)


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    Nikon inverted phase contrast microscope
    Inverted Phase Contrast Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inverted+phase+microscope/Inverted+Microscopes/pmc12963817-204-50-56
    Average 99 stars, based on 14654 article reviews
    inverted phase contrast microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Impact of enamel matrix proteins combined with collagen membranes on osteoblastic cell cultures.
    Article Snippet: Objective: Biodegradable collagen membranes are widely used in guided bone regeneration.. This study evaluated the effect of enamel matrix proteins (Emdogain®) combined with two collagen membranes (Bio-Gide® or Collprotect®) on osteoblastic cell cultures.. Methods: Human osteoblastic cells were cultured on Bio-Gide® or Collprotect® membranes coated with Emdogain® or left uncoated.

    Article Title: Thermo- and pH-Responsive Antimicrobial Hydrogels from Poly(2-(dimethylamino)ethyl methacrylate) and Cationic-Modified Chitosan for Sustained Papain Release
    Article Snippet: The cells were cultured in 25 cm2 flasks (Sarstedt) containing DMEM supplemented with 10% fetal bovine serum (Nutricell), 100 IU mL−1 penicillin (Sigma), and 50 μg mL−1 streptomycin (Sigma). .. The medium was refreshed every 2 days, and cell growth was monitored using an inverted phase microscope (Nikon Eclipse TS100). ..

    Article Title: Effects of desensitizing and/or anti-erosive toothpastes combined with mouthrinses on enamel structure and dental pulp fibroblasts viability.
    Article Snippet: .. Cell growth was daily monitored by using an inverted phase microscope (Nikon TMS, Nikon, Melville, NY, USA) and the medium was renewed every 2 or 3 days based on cellular metabolism. ..

    Article Title: Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture
    Article Snippet: .. After osteogenic differentiation (21 d), the culture plate was stained with Alizarin Red as described below and photographed under an inverted phase microscope (Nikon TS100F) using a 10x objective. ..

    Article Title: Thermo- and pH-Responsive Antimicrobial Hydrogels from Poly(2-(dimethylamino)ethyl methacrylate) and Cationic-Modified Chitosan for Sustained Papain Release
    Article Snippet: The cells were cultured in 25 cm 2 flasks (Sarstedt) containing DMEM supplemented with 10% fetal bovine serum (Nutricell), 100 IU mL –1 penicillin (Sigma), and 50 μg mL –1 streptomycin (Sigma). .. The medium was refreshed every 2 days, and cell growth was monitored using an inverted phase microscope (Nikon Eclipse TS100). ..

    Article Title: Enhancing transdermal delivery of chrysomycin A for the treatment of cutaneous melanoma and MRSA infections using Skin-Penetrating Peptide-Functionalized deformable liposomes.
    Article Snippet: Transdermal drug delivery represents a promising avenue for the treatment of dermatologic diseases, such as cutaneous melanoma and skin infections.. This study involves the development of a novel therapeutic strategy that employs a skin-penetrating peptide SPACE-modified flexible liposomal chrysomycin A (CA@SPACE-LP) with a particle size of 111.5 nm.. In vitro transdermal experiments demonstrated that CA@SPACE-LP was effective in enhancing the ability of the drug to penetrate the stratum corneum and enter deep into the skin tissue, increasing the intradermal drug concentration up to threefold compared to free CA.

    Article Title: Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture
    Article Snippet: .. After osteogenic differentiation (21 d), the culture plate was stained with Alizarin Red as described below and photographed under an inverted phase microscope (Nikon TS100F) using a 10x objective. ..

    Article Title: Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture
    Article Snippet: .. Finally, images were captured using an inverted phase microscope (Nikon TS100F, Japan) with a 10x objective. .. To quantify the deposition of calcium (or mineralization) in osteogenic differentiation, 250μL of extraction solution contatining 1% acetic acid and 1% methanol at a 4:1 proportion were added to each well previously stained with Alizarin Red; then, the plates were taken to the shaker (Orbital Shaker Form, Thermo, USA) for 30 min at room temperature.

    Staining:

    Article Title: Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture
    Article Snippet: .. After osteogenic differentiation (21 d), the culture plate was stained with Alizarin Red as described below and photographed under an inverted phase microscope (Nikon TS100F) using a 10x objective. ..

    Article Title: Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture
    Article Snippet: .. After osteogenic differentiation (21 d), the culture plate was stained with Alizarin Red as described below and photographed under an inverted phase microscope (Nikon TS100F) using a 10x objective. ..



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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Image Search Results


    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain

    Journal: BMC Oral Health

    Article Title: Dose-dependent effects of Metformin on proliferation and odontogenic differentiation of dental pulp stem cells

    doi: 10.1186/s12903-026-08221-w

    Figure Lengend Snippet: Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain

    Article Snippet: Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14.

    Techniques: Staining, Cell Culture, Control, Microscopy, Software, Derivative Assay